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Literature summary extracted from

  • Gupta, A.; Roy, I.; Khare, S.K.; Gupta, M.N.
    Purification and characterization of a solvent stable protease from Pseudomonas aeruginosa PseA (2005), J. Chromatogr. A, 1069, 155-161.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.26 1,10-phenanthroline 5 mM, 0% residual activity Pseudomonas aeruginosa
3.4.24.26 1,4-dithiothreitol 5 mM, 0% residual activity Pseudomonas aeruginosa
3.4.24.26 Ca2+ 5 mM, 54% residual activity Pseudomonas aeruginosa
3.4.24.26 cetyltrimethylammonium bromide 0.1%, 53% residual activity Pseudomonas aeruginosa
3.4.24.26 Cu2+ 5 mM, 21% residual activity Pseudomonas aeruginosa
3.4.24.26 EDTA 5 mM, 25% residual activity Pseudomonas aeruginosa
3.4.24.26 Mg2+ 5 mM, 56% residual activity Pseudomonas aeruginosa
3.4.24.26 Mn2+ 5 mM, 40% residual activity Pseudomonas aeruginosa
3.4.24.26 Ni2+ 5 mM, 12% residual activity Pseudomonas aeruginosa
3.4.24.26 sodium dodecylsulfate 0.1%, 61% residual activity Pseudomonas aeruginosa
3.4.24.26 Zn2+ 5 mM, 63% residual activity Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.26 additional information
-
 casein Km-value is 2.7 mg/ml Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.26 extracellular
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.26 35000
-
 x * 35000, SDS-PAGE Pseudomonas aeruginosa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.24.26 benzene 25% v/v, 30°C, stable for at least 72 h Pseudomonas aeruginosa
3.4.24.26 cyclohexane 25% v/v, 30°C, stable for at least 72 h Pseudomonas aeruginosa
3.4.24.26 hexane 25% v/v, 30°C, stable for at least 72 h Pseudomonas aeruginosa
3.4.24.26 toluene 25% v/v, 30°C, stable for at least 72 h Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.26
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.26 99610
-
 60°C, pH 8.0 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.26 ? x * 35000, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.26 60
-
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.26 60
-
 30 min, no loss of activity Pseudomonas aeruginosa
3.4.24.26 65
-
 10 min, 33% loss of activity Pseudomonas aeruginosa
3.4.24.26 70
-
 10 min, 80% loss of activity Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.26 8
-
-
 Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.26 6 9
-
 Pseudomonas aeruginosa